Conclusions Unexpectedly, the lesion area of the entire aorta

\n\nConclusions Unexpectedly, the lesion area of the entire aorta was reduced significantly in the AAV8-ASM virus-treated group. Hepatic expression and secretion of ASM into the circulation did not accelerate or exacerbate, but rather decreased, lesion formation in ApoE(-/-) mice. Thus, plasma ASM activity does not appear to be rate limiting for plaque formation during atherogenesis. Copyright (C) 2011 John Wiley & Sons, Ltd.”
“Voltage-gated calcium (Ca(2+)) channels

are thought to play an important role in epileptogenesis and seizure generation. Here, using the whole cell configuration of patch-clamp techniques, we report on the modifications of biophysical and pharmacological properties of high threshold voltage-activated Ca(2+) channel currents in inferior colliculus (IC) neurons of the genetically

Bcl-2 lymphoma epilepsy-prone rats (GEPR-3s). Ca(2+) channel currents were measured by depolarizing pulses from a holding potential of -80 mV using barium (Ba(2+)) as the charge carrier. We found that the current density of high threshold voltage-activated Ca(2+) channels was significantly larger in IC neurons of seizure-naive GEPR-3s compared to control Sprague-Dawley rats, and that seizure episodes further enhanced the current density in the GEPR-3s. The increased current density was reflected by both a -20 mV shifts in channel activation and a 25% increase in the non-inactivating fraction of channels in seizure-naive GEPR-3s. Such changes were reduced by seizure episodes in the GEPR-3s. Pharmacological analysis of the current density suggests that upregulation www.selleckchem.com/ALK.html of L-, N- and R-type of Ca(2+) channels may contribute to IC neuronal hyperexcitability

that leads to seizure susceptibility in the GEPR-3s. (C) 2008 Elsevier Ltd. All rights reserved.”
“Introduction: Many medical associations recommend nephron-sparing surgery (NSS) for tumours larger than 4 cm amenable to partial nephrectomy (PN). These recommendations are, however, mostly based on isolated reports. We systematically review the oncological outcomes of partial nephrectomy procedures performed for tumours larger than Cilengitide 4-cm. Methods: A PubMed search was carried out using keywords “partial nephrectomy” and “nephron sparing” for records dating back to 1995. In total, 2136 abstracts were analyzed; from these, 174 studies were scrutinized. We identified 32 manuscripts reporting size-specific cancer-specific survival rates for masses greater than 4 cm. From each of these studies, we recorded the number of PN, tumour diameter, follow-up duration, 5- and 10-year recurrence, overall and cancer-specific survival rates (OS, CSS). We also calculated weighted OS and CSS rates. Results: This systematic review includes 2445 patients with renal tumours larger than 4 cm who underwent PN: 1858 patients with tumours between 4 to 7 cm, 410 patients with tumours larger than 7 cm and 177 patients with tumours greater than 4 cm (exact size unknown).

The purpose of this study was to determine whether inhibition of

The purpose of this study was to determine whether inhibition of CaMKIV would improve disease pathology.\n\nMethods. We treated MRL/lpr mice with KN-93, a CaMKIV inhibitor, starting at week 8 or week 12 of age and continuing through week

16 and evaluated skin lesions, proteinuria, kidney histopathology, proinflammatory cytokine production, and costimulatory molecule expression. We also determined the effect of silencing of CAMK4 on interferon-gamma (IFN gamma) expression by human SLE T cells.\n\nResults. CaMKIV inhibition in MRL/lpr mice resulted in significant suppression of nephritis and skin disease, decreased expression of the costimulatory molecules CD86 and CD80 on B cells, and suppression of IFN gamma and tumor necrosis factor alpha production. In human SLE T cells, silencing of CAMK4 resulted in suppression of IFN gamma production.\n\nConclusion. PLX3397 mouse We conclude that suppression of CaMKIV mitigates disease development in lupus-prone mice by suppressing cytokine production and costimulatory molecule expression. Specific silencing of CAMK4 in human

T cells results in similar suppression of IFN gamma production. Our data justify the development of small-molecule CaMKIV inhibitors for the treatment of patients with SLE.”
“Objective: Acute encephalitis with refractory repetitive partial seizure (AERRPS) is a peculiar type of post-encephalitic/encephalopathic epilepsy. Here we report all AZD6094 cell line analysis of AERRPS in a series of children and propose an effective treatment option for seizure control in these children. Methods: We retrospectively reviewed cases of AERRPS treated in a pediatric intensive care unit., between February 2002 and June 2006. Clinical characteristics were systemically assessed. Burst Suppression coma was induced by high-dose suppressive therapy; 24-h electroencephalogram (EEG) monitoring was performed oil each patient. The goal of treatment was to achieve complete clinical seizure control or burst-suppression pattern on EEG, aiming for all interburst interval of >5 s. Brain imaging was done for each patient. Results: There were nine

patients click here (seven boys), aged 5-15 years. Clinical symptoms included fever (100%), upper respiratory symptoms (66.7%) and altered consciousness (66.7%). All patients received multiple high-dose suppressive drugs and were intubated with/without inotropic agents. Seizures in three patients were stopped after high-dose lidocaine infusion (6-8 mg/kg/h) in the acute stage and three patients were stopped after high dose phenobarbital (serum level 60-80 ug/mL) combined with high-dose oral topiramate (15-20 mg/kg/day). Follow-up for this study was 16-61 months. Two subjects died while seven developed epilepsy and/or neurologic deficits; none returned to baseline. All survivors were discharged and Continued multiple antiepileptic medications. Conclusions: Our data indicates that children with AERRPS have high mortality and morbidity rates.

Colocalization analysis suggested that fibronectin was a uniquely

Colocalization analysis suggested that fibronectin was a uniquely distributed matrix protein. Morphology, three-dimensional matrix adhesions, and integrin-mediated signaling during vasculogenesis were then studied in human endothelial cells seeded onto the fibroblast-derived matrix. Elongated morphology and decreased cell area were noted, as compared with cells on fibronectin-coated coverslips. Cell-matrix adhesions contained vinculin,

pY397-FAK, and pY410-p130Cas, and all of these colocalized more with fibronectin than tenascin-C, collagen 1, or collagen VI. Additionally, the endothelial cells remodeled the fibroblast-derived matrix and formed networks of tubes with demonstrable lumens. Matrix adhesions in these tubes also predominantly colocalized with fibronectin. The www.selleckchem.com/products/gsk2126458.html pattern of membrane type PCI-34051 1 matrix

metalloprotease expression in the endothelial cells suggested its involvement in the matrix remodeling that occurred during tubulogenesis. These results indicated that information in fibroblast-derived matrix promoted vasculogenic behavior. (C) 2009 Elsevier B.V. All rights reserved.”
“Background The purported advantage of lightweight large-pore meshes is improved biocompatibility that translates into lesser postoperative pain and earlier rehabilitation. However, there are concerns of increased hernia recurrence rate. We undertook a prospective randomized clinical trial to compare early and late outcome measures

with the use of a lightweight (Ultrapro) mesh and heavyweight (Prolene) mesh in endoscopic totally extraperitoneal (TEP) groin hernia repair.\n\nMethods A prospective study was performed on 402 patients (191 in Ultrapro and 211 in Prolene group) with bilateral groin hernias who underwent endoscopic TEP groin hernia repair from March 2006 HSP990 in vitro to June 2007. All operations were performed by five consultants following a standardized operative protocol. Chronic groin pain and hernia recurrence were evaluated as primary outcome measures. Secondary outcome measure were early postoperative pain, operative time, number of fixation devices required to fix the mesh, return to normal daily activities of work, seroma, and testicular pain.\n\nResults At 1-year follow-up, incidence in Ultrapro versus Prolene group for chronic groin pain was 1.6% vs. 4.7% (p = 0.178) and recurrence was 1.3% vs. 0.2% (p = 0.078). In Ultrapro versus Prolene group, mean visual analogue score for postoperative pain at day 7 was 1.07 vs. 1.31 (p = 0.00), mean return to normal activities was 1.82 vs. 2.09 days (p = 0.00), and mean number of fixation devices per patient required to fix the mesh was 4.22 vs. 4.08 (p = 0.043).\n\nConclusion Lightweight meshes appear to have advantages in terms of lesser pain and early return to normal activity. However, more patients had hernia recurrence with lightweight meshes, especially for larger hernias.

Gains were associated with age > 60 years, female gender, a tr

Gains were associated with age > 60 years, female gender, a trend for higher complete response rate, lower death rate, and better overall survival in patients treated with R-CHOP. Lesions were

inversely associated with bone marrow involvement and number of extra-nodal sites. Differentially expressed transcripts were enriched of genes belonging to specific pathways find more and miRNAs targets. MIR96, MIR182, MIR589, MIR25 were shown significantly up-regulated in 7q+ DLBCL by real-time PCR.”
“Fatty acid synthase (FASN) is the terminal enzyme responsible for fatty acid synthesis and is up-regulated in tumors of various origins to facilitate their growth and progression. Because of several reports linking the FASN and proteasome pathways, we asked whether FASN inhibitors could combine with bortezomib, the Food and Drug Administration-approved proteasome inhibitor, to amplify cell death. Indeed, bortezomib treatment augmented suboptimal FASN inhibitor concentrations to reduce clonogenic survival, which was paralleled by an increase in apoptotic markers. Interestingly, FASN inhibitors induced accumulation of ubiquinated proteins and enhanced the effects of bortezomib treatment. In turn, bortezomib increased fatty acid synthesis, suggesting

crosstalk between the pathways. We hypothesized that cell death resulting from crosstalk perturbation was mediated by increased unfolded protein response (UPR) signaling. Indeed, disruption of crosstalk activated and saturated the adaptation arm of UPR signaling, www.selleckchem.com/products/lb-100.html including eIF2 alpha phosphorylation, activating transcription factor 4 expression, and X-box-binding protein 1 splicing. Furthermore, although single agents did not activate the alarm phase of the UPR, crosstalk interruption resulted in activated c-Jun NH(2)-terminal kinase and C/EBP homologous protein-dependent cell death. Combined, the data support the concept

that the UPR balance between adaptive to stress signaling can be exploited to mediate Lonafarnib increased cell death and suggests novel applications of FASN inhibitors for clinical use. [Mol Cancer Ther 2008;7(12):3816-24]“
“Executive functions encompass various cognitive processes and are critical in novel or demanding driving situations. Our aim was to determine the role of impairments in specific executive functions (updating, flexibility, inhibition) on road performance in drivers with Parkinson’s disease (PD), a condition commonly associated with early executive dysfunction. In this pilot study, 19 patients with mild to moderate PD and 21 healthy controls matched for age, education, and driving experience were tested using a neuropsychological battery assessing global cognitive abilities, updating (n-back task), flexibility (plus-minus task), and inhibition (Stroop test).

” Initiatives with the greatest impact often cut across instituti

” Initiatives with the greatest impact often cut across institutional silos in colleges, departments, and programs, yielding measurable community health benefits. Community leaders also facilitated

collaboration by enlisting University of New Mexico educational and clinical resources to better respond to their local priorities. Early progress in New Mexico’s health outcomes measures and state health ranking is a promising sign of movement toward Vision 2020. (C) 2015 American Journal of Preventive Medicine”
“We designed and synthesized novel PPAR delta antagonists based Galunisertib datasheet on the crystal structure of the PPAR delta full agonist TIPP-204 bound to the PPAR delta ligand-binding domain, in combination with our nuclear receptor helix 12 folding modification hypothesis.

Representative compound 3a exhibits PPAR delta-preferential antagonistic activity. (C) 2009 Selleckchem NCT-501 Elsevier Ltd. All rights reserved.”
“Among the “omics”, glycomics is one of the most complex fields and needs complementary strategies of analysis to decipher the “glycan dictionary”. As an alternative method, which has developed since the beginning of the 21st century, lectin array technology could generate relevant information related to glycan motifs, accessibility and a number of other valuable insights from molecules (purified and non-purified) or cells. Based on a cell line model, this study deals with the key parameters that influence the whole cell surface glycan interaction with lectin arrays and the consequences on the interpretation and reliability of the results. The comparison between the adherent and suspension forms of Chinese Hamster Ovary (CHO) cells, showed respective glycan signatures, which could be inhibited specifically

by neoglycoproteins. The modifications of the respective glycan signatures were also revealed selleckchem according to the detachment modes and cell growth conditions. Finally the power of lectin array technology was highlighted by the possibility of selecting and characterizing a specific clone from the mother cell line, based on the slight difference determination in the respective glycan signatures.”
“MicroRNAs (miRNAs) are known to be involved in carcinogenesis and tumor progression in hepatocellular carcinoma (HCC). Recently, microRNA-7 (miR-7) has been proven to play a substantial role in glioblastoma and breast cancer, but its functions in the context of HCC remain unknown. Here, we demonstrate that miR-7 inhibits HCC cell growth and metastasis invitro and in vivo. We first screened and identified a novel miR-7 target, phosphoinositide 3-kinase catalytic subunit delta (PIK3CD). Overexpression of miR-7 would specifically and markedly down-regulate its expression.


“A pair of solvatochromic phenolate betaines that differed


“A pair of solvatochromic phenolate betaines that differed only in their lipophilicity was synthesized. Their solvatochromic responses in pure solvents, in a DMSO-MeOH solvent mixture as well as in micellar solutions were different, an observation which confirmed the fact that sensor lipophilicity contributes to the interpretation of solvatochromism. Quantum mechanical calculations reproduced the observed spectral differences. Molecular dynamics simulations shed light on the solute-solvent interactions responsible for their differences in solvent mixtures. (C) 2009

Elsevier Ltd. All rights reserved.”
“The weaver ant, Oecophylla smaragdina (Hymenoptera: Formicidae), is a successful predator and repellent of a range of insect pests of many economically important LCL161 crops and forest trees. To use the ant as a biocontrol agent, extension officers and farmers need to know the best time of day to identify and transplant

the ant colonies and to measure the abundance of the ant. To answer these questions, it is important to know about ant activity over a 24-h period at the colony level. Ant activities of three weaver ant colonies on the Tiwi campus of Charles Darwin University, Darwin, were measured in both dry and wet seasons in 1997 and 1998. The activity patterns on three types of ant trails see more showed that ants were least active between the hours of 10:30 and 14:00, and their activity peaked between 16:00 and 21:00 h. There was also a smaller activity peak from 8:00 to 9:00 h. The best time of day to identify ant colonies and to measure ant abundance is from 16:00 to 21:00 h (late afternoon to dusk). The best time to transplant weaver ant colonies Ubiquitin inhibitor is between 10:30 and 14:00 h (midday).”
“Since randomized controlled trials are difficult to perform for ethical reasons in a potentially deadly condition like status epilepticus (SE), a retrospective database analysis may be welcome to broaden the evidence for the treatment of SE. In this retrospective study we evaluated every SE treatment at the neurological department

of the University of Rostock from January 2000 to December 2009 in order to determine the efficacy of different antiepileptic drugs (AEDs) in terminating different kinds of SE. We analyzed the frequency of refractory courses in different types of SE, at which time which AED was administered and at which time which AED was effective to terminate the different epileptic conditions. A second aim of this study was to evaluate the course and the outcome of different kinds of SE. Statistical comparisons were performed with the x(2)-test. 167 episodes of SE in 118 patients could be evaluated. The efficacy rates of AEDs differed significantly, mainly due to the superior efficacy of clonazepam (CZP).

Clinical, radiological, biological

Clinical, radiological, biological RSL3 and therapeutic data and clinical course were studied. ResultsPositive anti-2-GPI antibodies were present in 28 patients. The predominant physiopathological process was mainly inflammatory (25% with myelitis, 14.3% with optic neuritis) or vascular (14.3% with cerebral ischaemia,

7.1% with cerebral vasculitis). Brain magnetic resonance imaging was performed in 89.3% of patients: atypical lesions were observed in 44% and typical inflammatory and vascular lesions in 16% and 12%, respectively. ConclusionThe anti-2-GPI antibody seems to be involved in two types of neurological disease: vascular or inflammatory multiple sclerosis-like’ disease. These two types of patients frequently develop an autoimmune disease (multiple sclerosis, systemic lupus erythematosus, APS). However, a large proportion of the patients had an undefined profile with aspecific cerebral lesions and required monitoring. This study raises questions about a separate entity at the border between APS and multiple sclerosis which remains to be better defined in a larger Src inhibitor cohort.”
“In Western countries, chronic hepatitis C virus (HCV)-infection mostly affects former and active substance

users. The effect of active substance use on interferon (IFN)-responsiveness and therapy efficacy is not well understood. In this study, we compared natural killer (NK) cell activity and function in healthy controls LY2606368 molecular weight and chronic HCV-infected patients with and without active substance

use, as well as the early effects of antiviral therapy with peg-IFN and ribavirin.\n\nNo differences were observed between chronic HCV patients and healthy individuals in the number and frequencies of CD56(dim) and CD56(bright) NK cells. Also, IL-12/18-induced IFN-gamma production by NK cells was comparable between all groups, whereas the cytotoxic ability of NK cells (granzyme and CD107a levels) was more potent in HCV-infected patients as compared to healthy controls, and highest in non-substance users. Moreover, at baseline, the activation of NK cells was significantly lower in HCV-infected patients who used substances, when compared to healthy individuals. Therapy-induced viral load reduction assessed early at day 7 showed a similar decline in substance users and non-substance use HCV patients, with 25% substance users and 17% non-substance users testing HCV-RNA negative at day 7. Furthermore, early during IFN-based therapy, NK cells from HCV patients remained responsive to IFN, and only a minor decline in the degree of STAT-1 phosphorylation was observed irrespective of substance use. These findings were further supported by comparable in vitro p-STAT-1 induction in all three experimental groups.

VEGF levels are elevated in peritoneal fluid and endometriotic

VEGF levels are elevated in peritoneal fluid and endometriotic

tissue from patients with endometriosis. In addition, it has been demonstrated that the expression of VEGF is potentiated by a variety of cytokines, including IL-1 beta. Recent studies show that leuprorelin reduces the production of VEGF-A and IL-1 beta in eutopic endometrial cell cultures, suggesting a mechanism by which it could inhibit the development of endometriosis. Thus, GnRH analogues appear to be effective in reducing the growth of endometrial cells, not only due GDC-0068 chemical structure to their classical pituitary endocrine effects, but also via a direct effect on the endometrial cells themselves. Copyright (C) 2008 S. Karger AG, Basel”
“Aldosterone this website receptor blockade and thiazide therapy effectively lower blood pressure in geriatric hypertension. Their impact on sympathetic nervous system function has not been evaluated. in a double-blind, randomized study, 36 patients with stage 1 hypertension underwent 6 months of therapy with either aldosterone receptor blockade (spironolactone, n=19; 68 +/- 1 years) or hydrochlorothiazide (n=17; 68 +/- 2 years). Arterial blood pressure, [(3)H]-norepinephrine (NE) kinetics (extravascular NE release rate), and alpha-adrenergic responsiveness (forearm vasoconstriction to graded intrabrachial artery NE infusions)

were evaluated at baseline, after a 4-week antihypertensive medication withdrawal, and after spironolactone or hydrochlorothiazide treatment. Arterial blood pressure decreased significantly

with both spironolactone (160 +/- 3 to 134 +/- 2 rum Hg; 77 +/- 2 to 68 +/- 2 mm Hg) and hydrochlorothiazide (161 +/- 4 to 145 +/- 4 mm Hg; 78 +/- 2 to 73 +/- 2 mm Hg) treatment. Sympathetic nervous system activity was significantly reduced after spironolactone (plasma NE: 378 +/- 40 to 335 +/- 20 pg/mL, P=0.04; [(3)H]-NE release rate: 2.74 +/- 0.3 to 1.97 +/- 0.2 mu g/min per meter squared, P=0.04) hut not hydrochlorothiazide (plasma NE: 368 +/- 25 to 349 +/- 23 pg/mL, P=0.47; [(3)H]-NE release rate: 2.63 +/- 0.4 to 2.11 +/- 0.2 mg/min per meter squared, P=0.21). alpha-Adrenergic responsiveness was unchanged with either drug treatment. These findings demonstrate a beneficial effect Repotrectinib clinical trial of aldosterone receptor blockade on reducing sympathetic nervous system activity and blood pressure in hypertensive older patients. (Hypertension. 2010;55:1217-1223.)”
“The prevalence, risk factors, and outcome of antibody-mediated rejection (AMR) of the kidney after simultaneous pancreas-kidney transplantation are unknown. In 136 simultaneous pancreas-kidney recipients who were followed for an average of 3.1 yr, 21 episodes of AMR of the kidney allograft were identified. Eight episodes occurred early (<= 90 d) after transplantation, and 13 occurred later.

Methods PPD skin test was performed in H 5 children with JIA

\n\nMethods. PPD skin test was performed in H 5 children with JIA who were

vaccinated with bacillus Calmette-Guerin (BCG), and then they were compared to the PPD response of 45 healthy children of the same age who were vaccinated with BCG. Children with a PPD induration >= 5 mm were accepted as PPD-positive. PPD induration >= 10 mm was accepted as a limit for suspecting tuberculosis.\n\nResults. PPD induration size and PPD positivity selleck products rates (PPD >= 5 mm) of children with JIA were significantly lower than those of healthy children. The mean of PPD induration size was significantly lower (p < 0.0001) in patients with either I BCG vaccine (3.7 +/- 3.6) or more than I BCG vaccine than controls see more with either

1 BCG vaccine (7.10 +/- 3.2) or more than I BCG vaccine (10.05 +/- 4.1). PPD was positive in 35.9% of patients with JIA vaccinated once (n = 32), in 50% of patients with JIA vaccinated more than once (n = 13), in 82.1% of controls vaccinated once (n = 23), and in 88.2% of controls vaccinated more than once. This result was statistically significant (patients, p = 0.03; controls, p = 0.039). It was determined that neither the activity of the disease nor the use of corticosteroid and methotrexate affected the PPD response.\n\nConclusion. The response to PPD, which is one of the Th1 cell-type responses, was significantly lower in BCG-vaccinated children with JIA compared

to healthy children. (First Release Aug 1 2009; J Rheumatol 2009;36:2029-32; doi:10.3899/jrheum.090173)”
“H5N1 avian influenza continues to be a potential pandemic threat. Several vaccine candidates based on potentially pandemic influenza strains and antiviral drugs have been tested in preclinical and clinical studies. The data obtained so far have shown some promise, but have also revealed some shortcomings with both of these approaches. We have identified and characterized an H5N1 neuraminidase-specific monoclonal antibody which specifically inhibits N1 neuraminidase SYN-117 activity of highly pathogenic avian influenza (HPAI) strains from clades 1 and 2. We have also shown the protective efficacy of this antibody in animal challenge models using homologous virus. Specific and effective inhibition of N1 NA could make this mAb a useful therapeutic tool in the treatment of human infection, in particular with oseltamivir- and zanamivir-resistant strains of HPAI.”
“Environmental, genotypic and agronomic factors have an effect on the yield value of an onion crop, which is determined primarily by number, weight and size of bulbs. Spatial variability of soil properties affects crop yield. Remote sensed hyperspectral vegetation indices (VIs), calculated using crop reflectance at field scale can be used either as an index of the plant biophysical status, or as a tool to estimate crop variability.

aerogenes has a large RNA population comprising 8 rRNA operons an

aerogenes has a large RNA population comprising 8 rRNA operons and 87 cognate tRNAs that have the ability to translate transferred genes that use different codons, as exemplified by the significantly different codon usage between genes from the core genome and the “mobilome.” On the basis of our findings, the evolution of this bacterium to become a “killer bug” with new genomic repertoires was from three criteria that are “opportunity, power, and usage” to indicate a sympatric lifestyle: “opportunity”

to meet other bacteria and exchange foreign sequences since this bacteria was similar to sympatric bacteria; “power” to integrate these foreign sequences such as the acquisition of several mobile genetic elements (plasmids, integrative conjugative element, prophages, transposons, flagellar assembly system, etc.) found in his genome; and “usage” Z-DEVD-FMK nmr to have the ability to translate these sequences including those from rare codons to serve as a translator of foreign languages.”
“BACKGROUND CONTEXT: Waitlists are commonly used in Canada to manage access to surgical procedures such as elective surgical lumbar discectomy (ESLD). The timing of enrollment onto the waitlist is important as this is a proxy measure for the concordance of preferences for surgery between a patient and

Smoothened inhibitor surgeon. After enrollment, the waiting time to actual surgery extends the duration of preoperative symptoms, which possibly affects the outcome of ESLD. Waiting time also specifically reflects the delay in service delivery imposed by the selleckchem limited capacity of the health-care system.\n\nPURPOSE: To determine if a system-imposed delay in treatment, that is, longer waiting time, for ESLD is associated with a higher odds of experiencing residual postoperative pain.\n\nSTUDY DESIGN/SETTING: Ambidirectional cohort study with 2-year retrospective and 3-year prospective components, conducted at a major tertiary care center serving a metropolitan area in Canada.\n\nPATIENT SAMPLE: Patients aged

16 years or older with sciatica because of herniated lumbar disc, confirmed on advanced imaging, were recruited at the time of waitlist enrollment for ESLD. Patients with significant comorbidity or emergency indications for surgery were excluded. Of 391 participants, 291 had complete follow-up information at 6 months postoperatively.\n\nOUTCOME MEASURE: Intensity of the predominant symptom (worse of either back or leg pain) was assessed on the 11-point numerical rating scale at waitlist enrollment and 6 months postoperatively. Pain scores were highly skewed and therefore categorized into four ordinal levels defined by quartiles.\n\nMETHODS: For the primary analysis, time to surgery from waitlist enrollment was dichotomized based on a predetermined clinically meaningful cut-point of 12 weeks. Ordinal logistic regression was used to compare the odds of experiencing higher pain intensity between wait groups.