Rabs are activated by specific guanine nucleotide exchange factors, which promote the release of GDP from Rab and binding of GTP to Rab, and the activated Rabs

are then inactivated by GTPase-activating proteins or spontaneously inactivated by their intrinsic GTPase activity [6], either of which terminates the cycle [6, 7]. Therefore, the identification and characterization of these Rab regulators, especially of GEFs, is crucial to understanding the spatiotemporal regulation of Rab GTPase activation. The small GTPase RAB-5, which is found at the plasma membrane and early endosomes, is a master regulator of early endocytic trafficking [8]. Like other small GTPases, RAB-5 is activated by an exchange of bound GDP with GTP, which is catalyzed by a family of guanine-nucleotideexchange

NVP-BGJ398 concentration factors. RABEX-5 was identified as an interactor of Rabaptin-5 and was found to possess GEF activity toward RAB-5 and related GTPases. Likewise, both Rabaptin-5 and RABEX-5 are essential for RAB-5-driven endosome fusion in vitro [9]. Aberrant RABEX-5 DNA Synthesis inhibitor expression may result in obstruction of the RAB-5-mediated endocytic vesicle fusion process, thereby causing defects in phagocytosis. Acalabrutinib solubility dmso The results showed that RABEX-5 was overexpressed in colorectal cancer and breast cancer [10, 11]. The data indicated that RABEX-5 may act as an oncogene that is involved in the formation and development of malignant tumors and might influence tumor biological behavior. However, the role and mechanism of action of RABEX-5 in prostate cancer have not yet been studied. In present study, we first analyzed the expression of RABEX-5 in

prostate cancer tissue by real time quantitative polymerase Baricitinib chain reaction. Subsequently, the association between RABEX-5 and prostate cancer clinicopathological factors was evaluated. Additionally, we assessed the influence of RABEX-5 mRNA expression on the biochemical recurrence free survival and overall survival of patients with prostate cancer. Tissue specimens A total of 180 human prostate cancer and paired adjacent noncancerous tissues were obtained from the second hospital of Tianjin medical university, which underwent radical prostatectomy at this hospital between 1999 and 2010 [12–14]. Written informed consent was obtained from all prostate cancer patients and this study was approved by the research ethics committee of Tianjin medical university (TMUhMEC2013011). This investigation conformed to the principles outlined in the Declaration of Helsinki. Demographic and clinicopathological data of prostate cancer patients were collected from medical records. None of the prostate cancer patients received androgen deprivation treatment, chemotherapy, or radiation therapy prior to radical prostatectomy. The tissue samples were snapfrozen in liquid nitrogen and stored at -80°C until used.