Presence of activated PSCs within the islets was assessed by immunohistochemistry(IHC) and immufluorescence(IF); á-smooth muscle actin; SMA by IHC&IF and glial fibrillary acidic protein(GFAP) by IF.Quantification of á-SMA was performed on flow cytometry for confirmation of intra islet PSCs. Transforming growth factor(TGF)-â gene expression within isolated islets was evaluated by RT-PCR. Results: Duration of symptoms was find more 1-2yrs
in non-diabetics and 3-10yrs in diabetic CP patients. Fibrosis was dense in the peri islet area as opposed to minimal intra islet fibrosis. While there was gross acinar cell degeneration, islet morphology was relatively maintained. Intra-islet á-SMA positive cells in CP patients without and with DM indicated presence of PSCs. FACS analysis of non â-cell islet suspension showed 0.1% and 1.1% á-SMA positive cells in non diabetic and diabetic CP respectively. Presence of intra-islet á-SMA and GFAP double positive cells confirmed intra islet activated PSCs. TGF-â mRNA was evident within the islets. Conclusion: TGF-â secreted by intra
islet activated PSCs may aid in differentiation of naïve and Th17 cells to IFNã secreting Th1 cells, thereby contributing to T-helper cell mediated inflammation and early â-cell dysfunction. Key Word(s): 1. chronic pancreatitis; 2. pancreatic stellate cells; 3. beta cell dysfunction; Presenting Author: YUAN FANGFANG Additional Authors: WEN QIANG, SU LEI Corresponding Author: YUAN FANGFANG Affiliations: Guangzhou General Hospital of Guangzhou Erismodegib concentration military command Objective: To investigate the correlation factors about the complications of the traumatic hepatic injury by nonsurgical management(NOM). Methods: The clinical date of 139 pationts with traumatic liver injury undergoing NOM treatment in our hospital from 2001 to 2011 were retrospectively analyzed based on the prognosis,
age, sex, the associated injuries, the variables clinical manifestation, the hepatic injury grade and motality compared between traumatic hepatic injury group without complication and traumatic hepatic injury complicated with complication group. The factors with learn more statistical significance were analyzed using logistic multivariate regression analysis. Results: 81 of 139 (58%) patients were undergoing NOM, survival rate was 100%. 9 pationts complicated with complications(11.1%),include Bile leakage(1.2%,1/81), infection(7.4%,6/81), infection combined with hemorrhage(2.4%,2/81).The factors adopted in the statistics included penetrating trauma(75, 24/81), combine peri-liver vascular injury (14.8%, 12/81), shock (27.1%,22/81), injury grade (I 23.4%, II 41.9%, III 40.7%, IV 4.9%, V 2.4%), amount of hemoperitoneum(mass 18.