Finally, whole genome
sequence analysis of our strain allows us to fully characterize this new species including the genetic determinants associated with its specific antibiotic resistance phenotype likely acquired from different sources. In silico DNA-DNA hybridization of the genome of CF Microbacterium yannicii against the two other available genomes (Microbacterium testaceum StLB037 and Microbacterium laevaniformans OR221) was very low (≤ 70%). This was similar to DNA-DNA hybridization experiments reported in the seminal paper on the description of Microbacterium yannicii G72T species by Karojet et al. who showed a genetic relatedness of only 15.9%, 31.2%, and 45.1% between reference strain Microbacterium yannicii G72 and Microbacterium hominis, Microbacterium insulae, and Microbacterium trichothecenolyticum, this website respectively . As all the organ transplant recipients, our patient was immunocompromised, with an over immunosuppressive regimen containing a long macrolide therapy in the context of chronic lung allograft dysfunction, such conditions
with might play a crucial role in the development of Microbacterium spp. infection or colonization. Indeed Microbacterium spp. have been described as a causative agent of infections in immunocompromised patients such as, cancer almost patients [28, 29], endophthalmitis patients , interstitial pulmonary infection after heart transplantation GANT61 solubility dmso , bone marrow transplant recipients , and bacteremia [32–34]. To the best of our knowledge, such infection with Microbacterium spp has not been previously described in the double context of lung transplantation and in cystic fibrosis. Microbacterium spp. have been isolated from
clinical specimens including blood culture, superficial wounds, pleural fluid, sinus aspirate, bone infection, endophthalmitis, dialysis fluid, lymph node, catheter tip, knee find more puncture fluid, wound swab, urine, gall bladder, throat swab, prosthetic hip infection, conjuctival swab, tracheal secretion and urethral swab . The source of this bacterium in our patient was also undetermined but in our opinion, plants or vegetables may be a potential source of transmission in CF patients as well as a possible person to person transmission from another patient. Bacteria of the genus Burkholderia, Pandoraea, or Pseudomonas for example, which are known to be frequently recovered in the respiratory tract of CF patients, are also endophytic bacteria in plants. There results reinforce the hypothesis that plant associated environments may act as niche for putative opportunistic human pathogenic bacteria .