On average, selleck chemicals treatment with 10% serum for 48 hours resulted Inhibitors,Modulators,Libraries in a six fold increase in the total number of cells in the wound and in the proliferated cells at both the edge and in the wound. In addition, there was an effect of time in the outer zone cells, with the total number of cells and the proliferated cells in the wound being greatest at 48 hours. No differences were detected in cells that migrated but did not prolifer ate in the wound of outer zone cells. The effects of IL 1 on inner and outer zone micro wound repair IL 1 treatment of meniscal cells from the inner or outer zones resulted in decreased accumulation of proliferated cells in the micro wound. As compared to the inner zone control at 48 hours, the overall total number of cells in the wound Inhibitors,Modulators,Libraries and the pro liferated cells in the wound were significantly decreased by IL 1 treatment.

However, 0. 1 ng mL IL 1 at 48 hours showed an increase in the total cells in the wound, as compared to all other treatments at 24 hours, and a corresponding increase in the number of cells that migrated but did not proliferate in Inhibitors,Modulators,Libraries the wound, as compared to all other treatments at both 24 and 48 hours. There was a signifi cant increase in the number of migrated cells in the wound at 48 hours in the 1 ng mL and 10 ng mL IL 1 treatment groups. Overall for inner zone cells, the control treatment caused the greatest proliferation at the edge and in the wound and decreased the number of cells that migrated but did not proliferate in the wound. There was also an effect of Inhibitors,Modulators,Libraries time, with 48 hours showing increased total cells, proliferated cells and migrated cells in the wound, as compared to the 24 hour time point.

In the outer Inhibitors,Modulators,Libraries zone meniscal cells, IL 1 treatment caused a significant decrease in the number of prolifer ated cells in the wound, as compared to control. However, IL 1 did not have a significant effect on the total cell numbers in the wound, migrated cells in the wound, or the proliferated cells at the edge in the outer zone meniscal cells. The effects of TNF a on inner and outer zone micro wound repair Meniscal cells from the inner, but not the outer zone, showed diminished accumula tion of proliferated cells in the micro wound with increasing concentrations of TNF a. In the inner zone cells, proliferation at the edge was diminished by all concentrations of TNF a, as com pared to control. In addition, the 1 and 10 ng mL con centrations of TNF a caused significant decreases in proliferation at the edge, as compared to 0. 1 ng mL TNF a. At 48 hours, proliferation in the wound was significantly higher than at 24 hours. In the inner zone cells treated compound libraries with TNF a, there were no differences in the total cells in the wound or migrated cells in the wound.