7 days (Cader et al 2010). A total of 86 participants (43 per group) would provide 80% power, at the two-sided 5% significance level, to detect a difference of 24 hours between the experimental and control groups as statistically significant. Continuous data were summarised

as means and standard deviations (SD). Categorical data were summarised as percentages. To compare the same variable at different time points within each group, a two-way ANOVA was used. Differences in relation to the mechanical ventilation period, controlled ventilation period, and the weaning period between groups were compared with a Student’s t test. Mean differences (95% CI) between groups are presented. Chi-square (χ2) test was used for categorical variables. Data were analysed by intention to treat with a significance BGJ398 in vivo level of p < 0.05. Recruitment and data collection were carried out between March 2005 and July 2007. During the recruitment period, 98 Libraries patients were screened for eligibility. Of the 98, four patients were excluded from the study because of haemodynamic Selleckchem CHIR 99021 instability and two other patients were excluded because of a confirmed diagnosis

of neuromuscular illness. Ninety-two patients met the eligibility criteria and were randomised: 45 to the experimental group and 47 to the control group. The baseline characteristics of the patients are presented in Table 1 and in the first two columns of Table 2. One participant in each group was tracheostomised before extubation. Two participants in the experimental group and five in the control group died before extubation. Four participants in the experimental group and two in the control group required cessation of the weaning process and returned to controlled ventilation before extubation.

This decision was based on the physician evaluation that the participants had haemodynamic and/or respiratory deterioration requiring vasoactive drugs and/or sedative agents. Seventy-seven participants completed the weaning period (38 in the intervention group and 39 in the control group). The flow of participants through the trial is illustrated in Figure 1. The intensive care unit had a total of 28 adult medicalsurgical beds. The physiotherapy team consisted Mephenoxalone of four physiotherapists working in two shifts, all with expertise in intensive care. The Intensive Care Unit of Hospital de Clínicas in Porto Alegre, Brazil, was the only centre to recruit and test patients in the trial. Participants in the experimental group underwent training daily throughout the weaning period. The load trainingwas 40% of maximal inspiratory pressure and showed an increase in all patients in the experimental group. The initial load was 13 cmH2O (SD 5) and the final load of was 16 cmH2O (SD 5).