Was 23 tiPlant was deprived of nitrogen on day 3 was 2.3 times h Ago as nitrogen in plants abound. Anthocyanins are not detectable 3-Methyladenine in the samples used in the growth conditions. Discussion If you start in in vitro enzyme assays, the substrates on the basis of previous results on substrates for F3, 5 H enzymes from other plants were accepted weight Hlt. The substrates were also on the basis of the structural Selected similarity with these compounds Hlt is. Liquiritigenin took exception substrates metabolized by CYP75A31 were also found to be metabolized by CYP75A8, the previously isolated from C. roseus. Kaltenbach group also tested a petunia F3 5 H in the system used for E. coli expression CYP75A8, and found that the petunia F3 5 H accepts the same substrates.
C. Adu Supply F3 roseus, 5 H had h Activity here T with apigenin, petunia F3, 5 H had h Activity here T with naringenin. The enzyme CYP75A31 there was a clear Pr Conference for naringenin and Liquiritigenin because these substrates were also microsomal pr Rutoside Metabolized diluted preparations. In this study, CYP75A8 was also in the same yeast system there CYP75A31 expressed. Km naringenin was measured at 1.20 M for CYP75A31, CYP75A8 and 0.83 M. Kaltenbach et al. reported an apparent M naringenin 7 km from CYP75A8 expression in the expression system in E. coli. The rate of hydroxylation by F3, is performed is 5, uses a function of H-reductase enzyme in the expression system. Of Vetten et al. showed that cytochrome b5 for the full activity F3, 5 H t required in petunia.
The gene inactivated by a cytochrome b5 transposon mutagenesis target, then causes a reduction in F3, 5, H and accumulation of reduced activity t of 5 substituted anthocyanins, leading to a Verf Coloring the flower. Our expression studies using the Arabidopsis reductase ATR1, w While expression studies by Kaltenbach et al., C. roseus was cytochrome P450 reductase in the expression system using E. coli. The use of different expression systems, and can make the difference in reductases Km values for the enzyme CYP75A8 C. roseus obtained in both studies. Liquiritigenin our knowledge has not been shown to be metabolized by an F3, 5 H enzyme before. Liquiritigenin in plants. Mostly with legumes which have a power isomerization CHI 6, hydroxy, and 6 to 5 deoxychalcones deoxyflavanones hydroxy and 5 are assigned to Joung et al.
noted that CHI tobacco. able to 6 isoliquiritigenin deoxychalcone deoxyflavanone 5, in transgenic tobacco Liquiritigenin overexpressing chalcone reductase gene Pueraria montana is isomerized Tanaka et al. showed that the F3, 5, H Gentiana triflora hydroxylation of naringenin for eriodictyol, eriodictyol to 5, 7, 3, 4, 5, pentahydroxyflavanone, dihydrokaempferol catalyzes dihydroquercetin, dihydroquercetin of apigenin and luteolin dihydromyricetin when expressed in S. cerevisiae under the control Promoter of glyceraldehyde-3-phosphate dehydrogenase. Stored reaction rates and substrate preferences Pr Into bacteria or yeast expression systems are not necessarily the actual product chliche rate or pr Planta conferences. As shown in this study, tomatoes F3, 5 is H Liquiritigenin able to metabolize, although our Knowle.