Sequence tags in
the database PASSIOMA. AndMethods 2.Material 2.1. Search TER counterpart HIF Signaling Pathway Passiflora anthocyanin biosynthesis genes. Tags genes clustered database project PASSIOMA expressed as a main source of data for the analysis were used. These sequences were obtained from EST sequences from the lacing of several mounted P. edulis or P. cDNA libraries suberosa of flower buds in various stages of development. The nucleotide and deduced amino acid Acid sequences of the genes known to have been involved in the biosynthesis of anthocyanins obtained fromthe nation al Center for Biotechnology Information. Research putative homologous sequences in the database using TBLASTN PASSIOMA module that contains the consensus amino compares Acid sequence with a database of nucleotide sequences were translated.
We usually Arabidopsis thaliana as Petunia hybrida or consensus sequences of the query that the biosynthesis of anthocyanins in these model types are investigated in detail at the molecular level. All sequences in the database, which showed a significant alignment with the query PASSIOMA were extracted from the database PASSIOMA. Clustering of all readings was identified with a given query sequence. CAP3 with the algorithm of the software BioEdit New ammunition obtained consensus sequences for the presence of conserved motifs using InterProScan andwere compared retested to databases of NCBI BLAST. Sequences that do not are the main reasons that have been rejected in the query sequence. Validated sequences were then included in the phylogenetic analyzes.
2.2. Comparison of the amino acid Acid sequences and phylogenetic analysis. All amino acid sequences aligned With the ClustalX software were defaults. Alignments were eventually corrected by hand Lich and genetics in the software analysis of molecular evolution. Phylogenetic B Trees were. With thrift and / or calculations of genetic distance Neighbor joining and bootstrap B Trees were also built. Third Results The project PASSIOMA cDNA obtained from mRNA extracted from flower buds at different stages of development, and it is expected that all ESTs corresponding genes w Expressed during flower development Passiflora. This research identified a total of 75 ESTs sequence Passiflora, 34 of them corresponding to P. edulis sequences and 41 of them corresponding sequences derived from P.
suberosa libraries. When CAP3 algorithm and a detailed comparison of their deduced amino Subjected acid sequences, the number of valid clusters was reduced to 15, m May receive corresponding to 15 different genes. When the amino acid sequences Validated database PASSIOMA other sequences derived from plant proteins Were compared in Public databases, the first explosion hits corresponded general sequences Populus and Ricinus. This result was expected, since these species Passiflora and the same order go Ren and are considered closely related. CHS, DFR, GT, GST, MYB and WD40 We ESTs corresponding genes in the family genes were assembled. Therefore, we have 15 Passiflora assembled sequences of the PA .
Sequence tags in