The suitability of Lu-177 for biomedical applications was ascertained by labeling 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid D-Phe(1)-Tyr(3)-octreotate(DOTA-TATE) with Lu-177.
Results: This process could provide NCA Lu-177 with >99.99% radionuclidic purity and an overall separation yield of similar to 99% was achieved within 3-4 h. The Hg content in the product was determined to be <1 ppm. Radiolabeling yield of >98% was obtained with DOTA-TATE under the optimized reaction conditions.
Conclusions: An efficient strategy for the separation of NCA Lu-177, suitable for biomedical applications,
has been developed. (C) 2010 Elsevier Inc. All rights reserved.”
“A technetium-99m-labeled
derivative from sulfanilamide, further referred to as Tc-99m-N-SFC, targeting infections BI 2536 in vivo in experimental animals, has been synthesized. The biological features of this radioactive agent have also been studied. The N-sulfanilamide ferrocene carboxamide (N-SFC) was chemically synthesized and then labeled with technetium-99m. It has been confirmed through this work that it is stable and obtained with radiolabelling yield (>87%). Radiochemical selleck chemicals analyses of Tc-99m-N-SFC revealed that the molecule was labeled rapidly (within 2 min) and effectively with little free pertechnetate in the preparations containing purified compound. Furthermore, in vitro investigations were conducted and the label’s stability in serum was observed up to 24 h of testing. Uptake of the tracer with live and heat/killed bacteria was compared in physiological conditions and was about 69% and 61.9% for the Escherichia coli and Staphylococcus aureus strains, respectively. We concluded that synthesis and labeling of Sulfanilamide
derivative with Tc99m- by this method is rapid, efficient and safe. Biodistribution studies demonstrated that our radiolabeled compound is accumulated rapidly and significantly Cyclin-dependent kinase 3 (P<.05) at infection sites. The comparison of the Tc-99m-N-SFC accumulation at sites of S. aureus-infected animals, which is expressed as target-to-non-target ratio, (2.88+/-0.10) with other radiotracers was discussed. (C) 2010 Elsevier Inc. All rights reserved.”
“Introduction: High specific radioactivity is preferable in the measurement of neuroreceptor bindings with positron emission tomography (PET) because receptor occupancy by mixed cold ligand hampers the accurate estimation of receptor binding. Recently, we succeeded in synthesizing [C-11]raclopride, a dopamine D, receptor ligand, with ultra-high specific radioactivity, i.e., several thousand GBq/mu mol. In the present study, we compared the [C-11]raclopride bindings to dopamine D-2 receptors between radioligands with ultra-high specific radioactivity and ordinary high specific radioactivity in healthy human subjects.