In contrast overproduction of FabB has the opposite result; unsaturated fatty acid levels are increased [25]. However, if the two enzymes are simultaneously overproduced, the fatty acid

composition returns to normal [25]. These counter-intuitive results are due to the fact that FabA catalyzes reversible reactions whereas the FabB reaction is irreversible. Hence, when FabB activity is limiting, any excess cis-3-decenoyl-ACP produced by FabA can be isomerized back to trans-2-decenoyl-ACP and upon FabI action, this acyl chain can enter the saturated arm of the pathway. However, when FabB is in excess, it catalyzes the irreversible elongation of cis-3-decenoyl-ACP and thereby pulls the flow of carbon toward the unsaturated branch of the pathway. Thus, it would seem a surprising finding if the C. acetobutylicium FabF was able to accurately partition acyl chains AZD1390 between the two branches of the fatty acid synthetic pathway of a foreign organism. It should be noted that it was not unexpected that the FabF homologue encoded within the fab gene cluster was the only FabF homologue that functioned in fatty acid synthesis. There are good arguments against the other two homologues having this function. The CAC2008 ORF in located within a cluster of genes that appear involved https://www.selleckchem.com/products/ve-822.html in synthesis

of a glycosylated product of a hybrid polyketide-nonribosomal polypeptide pathway. If so, the CAC2008 ORF would be involved in synthesis of the polyketide moiety. The CAA0088 ORF is encoded on the C. acetobutylicium

megaplasmid required for the late steps of solvent production by this organism. C. acetobutylicium survives loss of the megaplasmid [26] and therefore the CAA0088 ORF cannot encode an enzyme essential for fatty acid synthesis (although it could still provide FabF function). Note that it has been recently reported that the single FabF protein of the distantly related gram positive bacterium Lactococcus lactis can Gefitinib research buy also SN-38 perform the FabB reaction as well as that of FabF[27]. Conclusion Unsaturated fatty acid synthesis in Clostridia cannot be explained by a plenipotent FabZ indicating that these bacteria encode a novel enzyme that introduces the cis double bond. In contrast the Clostridia FabF protein has the functions of both of the long chain 3-ketroacyl-ACP syntheases of E. coli. The diversity of bacterial enzymes used for synthesis of the cis double bond of unsaturated fatty acids is unexpected because the remainder of the fatty acid synthetic enzymes is well conserved among very diverse bacteria. Methods Bacterial strains, plasmids and growth conditions The E. coli strains and plasmids used in this study are listed in Additional file 1. Luria-Bertani medium was used as the rich medium for E. coli. The phenotypes of fab strains were assessed on rich broth (RB) medium [12]. Oleate neutralized with KOH was added to RB medium at final concentration of 0.