Even if this were not true, light coupling into the slit and propagation though it would make the field behind the exit plane of the probe virtually symmetric about the z axis. Therefore, also the field amplitude distributions in the focal region are virtually independent

on the position of the incident field; only the measured intensity changes and therefore allows the profiling of 17-AAG in vitro the incident field without moving the detector. Figure 9a shows a comparison of the magnetic intensity profile |H y |2 of the incident field and the click here result of simulated measurement through the probe under conditions that approximate our experimental setup. For the convenience of resolution judgment, the peak values of both profiles have been normalized to unity, and the profiles are identical almost within the plotting precision. The simulated measured profile is slightly wider than the true incident field owing to the finite width of the slit. A normalized plot of simulated measurement without the corrugations in the probe gives a profile indistinguishable from the red curve in Figure 9a. However, the advantage of having the corrugations is obvious from Figure 9b. Here, we compare the peak values of the measured signal with and without the corrugations as a function of the numerical aperture of the collection optics. Without

the corrugations, the beaming effect disappears, and hence, the sensitivity gain for small numerical apertures Topoisomerase inhibitor is Fludarabine clinical trial as high as 3 to 4. At NA=1.4, which corresponds to our experimental setup, the theoretical gain factor is still approximately 1.5. Figure 9 Simulated transmittance. (a) Magnetic field intensity of the incident beam at the entrance plane of the probe (black line) and the simulated measurement result (red line), normalized to have a unit peak value. (b) Dependence of

the sensitivity gain factor achieved by having the corrugations in the probe, plotted as a function of the collection NA. Scanning electron micrographs of the device taken during the fabrication process are presented in Figure 10a and in the inset Figure 10b, where the grating-glue interface and the slit in the aluminum film are shown, respectively. In Figure 10a, the glue was partially peeled off from the Al layer (on the bottom of the figure) due to cutting of the structure for cross-sectional imaging, but high-accuracy penetration into the grooves is visible from the modulation. The slit shown in Figure 10b is not etched completely through; hence, a longer etch time was used to fabricate the final probe. The inset of Figure 10c shows the AFM image of the top surface without the TiO2 layer to illustrate the high-quality metal surface obtained by the template stripping process.