1 M PB, and then immersed in 30% sucrose solution until BEZ235 molecular weight it sank. Tissues were sectioned on a sliding microtome at 40-μm thickness. Every sixth serial section was selected and processed for immunostaining. The primary antibodies used were against mouse CD11b (1:400), NeuN (1:500), C/EBP-α (1:300), and C/EBP-β (1:300). The following day, brain sections were rinsed with PBS 0.5% BSA and incubated with appropriate secondary antibodies. The immunoreactive signals were observed using Alexa Fluor® 488 goat anti-mouse and Alexa Fluor® 594 goat anti-rabbit (1:200) and viewed by confocal

microscopy capture imaging. The results are presented as mean ± standard error of the mean (SEM). All analyses of variance were followed by Fisher’s least significant

difference posthoc analyses. Statistical significance was set at p < 0.05. The authors thank the Department of Education and Research, Taichung Veterans General Hospital for the Alvelestat excellent editing and technical assistance. This work was supported by grants from Taichung Veterans General Hospital, Taiwan (TCVGH-977304B) and the National Science Council of Taiwan (NSC96-2320-B-040-003-MY3 and NSC-101–2314-B-075A-003-MY2). The authors declare no financial or commercial conflict of interest. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the

authors. Figure S1. IL-13 reciprocally regulated COX-2, PPARγ, and HO-1 protein expression in a dose-dependent manner. Figure S2. (A) Quantitative analysis of the activated caspase-12 (cleavage Rho of pro-caspase-12) in BV-2 microglia protein expression, by densitometry (Image-Pro Plus software) (n = 4). # p < 0.05, compared to LPS groups. Figure S3. IL-13 regulated LPS-induced C/EBPα and C/EBPβ translocation. Figure S4. Representation of distribution of methylene blue dyes for different infusion times. Figure S5. Representation of distribution of methylene blue dyes for different infusion times and assessment of neurobehaviour in water maze. "
“We have previously demonstrated that the anti-inflammatory prostaglandin 15-deoxy-Δ 12,14-prostaglandin J2 (15dPGJ2) delays inflammation-induced preterm labour in the mouse and improves pup survival through the inhibition of nuclear factor-κB (NF-κB) by a mechanism yet to be elucidated. 15dPGJ2 is an agonist of the second prostaglandin D2 receptor, chemoattractant receptor homologous to the T helper 2 cell (CRTH2). In human T helper cells CRTH2 agonists induce the production of the anti-inflammatory interleukins IL-10 and IL-4. We hypothesized that CRTH2 is involved in the protective effect of 15dPGJ2 in inflammation-induced preterm labour in the murine model.