5% (19 of 767) of those in the 1980–1992 period (P<0.0001). Multivariable analysis confirmed the following independent predictors of higher odds of non-B infection: African ethnicity, heterosexual Navitoclax order route of infection and later time of diagnosis (Table 2). A broad heterogeneity of the 417 non-B group M clades was found in patients regardless of their different country of origin. All known pure subtypes, with the exception of K, plus seven distinct CRFs (01, 02, 04, 06, 09, 12 and 13), were detected. The most prevalent pure subtypes were F [n=99 (23.7%); 98

F1 and one F2], A [n=53 (12.7%); 38 A1, three A2 and 12 A3], C (n=47; 11.3%) and G (n=23; 5.5%). Among CRFs, CRF02_AG and CRF01_AE were the most frequent forms [n=107 (25.7%) Quizartinib cell line and n=21 (5.0%), respectively]. Thirty-nine URFs (9.3%), showing complex mosaic patterns, were identified. The distribution of non-B subtypes differed markedly between patients of European and African origin (n=192 and 146, respectively) (data not shown). The F1 subtype, which was present only in one African individual, was the most frequent clade in Europeans with non-B variants

(85 of 192; 44.3%), while the prevalences of A1 (n=24), C (n=19), CRF02_AG (n=9) and URFs (n=19) were 12.5, 9.9, 4.7 and 9.9%, respectively. European patients carrying the F1 subtype were mainly Italians (n=68; 82%) and Romanians (n=13; 15.7%). Among Europeans carrying non-B subtypes, 64.8% (n=57) were heterosexual MTMR9 and 74.5% (143 of 192) were male. An association between heterosexual route of infection, but not gender, and non-B clades was found in this group of subjects

(P<0.0001 and P=0.46, respectively). Differences in the distribution of subtype B vs. individual non-B clades were then analysed for non-B clades detected at a prevalence of >5%. A significant association with heterosexual route of infection was detected for subtypes F1 and C, with 50% of F1-infected (17 of 34), 100% of C-infected (six of six) and 30.6% of B-infected (528 of 1724) patients being heterosexual (P=0.006 for F1 vs. B; P<0.001 for C vs. B; P=0.026 for F1 vs. C). No association with gender was detected for any individual clade in Europeans. Among Africans living in Italy, CRF02_AG was found in 52.1% of subjects (n=76), followed by C (n=15; 10.3%), A [10 A3 (6.9%) and six A1 (4.1%)], G (n=13; 8.9%) and B (n=13; 8.2%) clades and URFs (n=10; 6.9%). Country of origin was known for 102 of these patients. Percentages of immigrants from Ivory Coast, Nigeria, Cameroon and Senegal were 21.6, 21.6, 12.7 and 9.9%, respectively. The remaining individuals (34.3%) were from northern (n=9), western (n=9), eastern (n=10), central (n=5) and southern Africa (n=2). Ninety-six (93.2%) of these patients were heterosexual and the male to female ratio was about 0.5:1 (36:65). Twenty out of 98 (20.4%) Latin American patients (52.9% from Brazil, 15.