Optimal pit and fissure sealing is determined by surface preparation techniques and choice of materials. Aim.  This study Sotrastaurin cost aimed (i) to compare the microleakage and penetration depth of a hydrophilic sealant and a conventional resin-based sealant using one of the following preparation techniques: acid etching (AE) only, a diamond bur + AE, and Er:YAG laser combined with AE, and (ii) to evaluate the microleakage and penetration depth of the hydrophilic pit and fissure sealant on different

surface conditions. Design.  Eighty recently extracted 3rd molars were randomly assigned to eight groups of ten teeth according to the material, preparation technique, and surface condition. For saliva contamination, 0.1 mL of fresh whole human saliva was used. All samples were submitted to 1000 thermal cycles and immersed in 2% methylene blue dye for 4 h. Sections were examined by a light microscope and analysed using image analysis software (Sigmascan®). Results.  The combination of Er:YAG + AE + conventional sealant showed the least microleakage. The sealing ability of the hydrophilic sealant was influenced BKM120 solubility dmso by the surface condition. Conclusion.  Er:YAG ablation significantly decreased the microleakage at the tooth–sealant interface compared to the non-invasive technique. The hydrophilic sealant applied on different surface conditions showed

comparable result to the conventional resin-based sealant. “
“Caries is a major oral health problem children with efforts focused on promoting use of caries prevention methods. The aim of the study is to assess the effect of a school-based oral health education programme on use of oral self-care measures for reducing caries. A structured school-based

oral health education programme was implemented in six schools in Ile-Ife, Nigeria for 4 years. At the end of the project, information was sought from school children in their last year and final 2 years of studies on the use of fluoridated toothpaste, consumption of sugar-containing snacks more than once a day, frequency of tooth brushing and flossing, and Progesterone time of the last dental check-up. Predictors of the use of preventive oral health practices for caries were determined. School children who received the intervention were more likely to report frequent use of fluoride-containing toothpastes (P < 0.001), more likely to brush twice a day (P = 0.03), less likely to consuming sugar-containing snacks less than once a day (P = 0.03) and less likely to use dental floss once a day (P < 0.001) when compared to the control group. This long term school based educational programme was able to increase school children's use of fluoride-containing toothpaste and twice daily tooth brushing, which are critical tools for reducing the risk of caries. "
“International Journal of Paediatric Dentistry 2012; 22: 331–341 Aims.