Taken, collectively, the data suggest that curcumin concentration dependently induces THP 1 cell apoptosis by each the extrinsic and intrinsic apoptotic pathways. Apoptosis of THP one cells by curcumin is just not mediated by PI3K AKT pathway PI3K AKT FOXO pathway is renowned for regulation of cell survival and apoptosis. Nonetheless, no synergistic or additive effect was observed when these two inhibitors had been mixed, implying that JNK and ERK may well be redundant in this technique. Con sistently, Inhibition of ERK lowered the phosphorylation of ERK, JunB and, to a lesser extent, c Jun. In sharp contrast, Inhibition of JNK lowered the phos phorylation of JNK and c Jun. Apart from, the percentage of sub G1 population in THP one cells handled with vehicle and curcumin with devoid of the inhibitors of ERK, JNK or the two was assessed employing DNA written content assays.
Curcumin drastically selleck inhibitor improved the percentage with the sub G1 population of THP 1 cells. This sub G1 population induced by curcumin was even more lowered from the inhibitor of ERK and JNK. A more pronounced reduction in sub G1 population was observed in THP one cells treated with combinational inhibitors. The data about the reduction of cur cumin mediated THP 1 cell apoptosis from the MAPK inhibitors making use of DNA material assays is steady with individuals obtained from capase three seven assays. Total, the information suggest that curcumin modulates apoptosis in THP one cells via the activation of JNK ERK Jun pathways. ERK and JNK pathways might be parallel and redundant within the curcumin induced THP 1 cell apoptosis.
PMA therapy lowers curcumin induced THP 1 cell apoptosis selleck chemicals MG-132 by inhibiting ERK JNK Jun pathways PMA is known to induce differentiation of THP 1 monocytic cells into macrophage like cells. Up coming, we compared the effect of curcumin on PMA handled THP one cells, differentiated mature mono cytic cells, and THP 1 cells making use of WST 1 assays. We found that cell viability of PMA taken care of THP one cells and THP one cells just after curcumin treatment method was 25 0. 5% and 96 three. 7%, respectively. The information propose that PMA therapy substantially reversed curcumin induced THP1 cell death. Up coming, we examined the result of curcumin over the ERK JNK Jun, caspase three and AKT pathways in PMA taken care of THP 1 cells. We located that curcumin decreased the phosphorylation of ERK, JNK, c Jun and JunB as well as the degradation of caspase 3 in PMA taken care of THP one cells as opposed to THP one cells. In contrast, curcumin greater the phosphorylation of AKT. The data showed that PMA therapy reversed the apoptotic result of curcumin on THP one cells through the inactivation of ERK JNK Jun pathways and activation of AKT pathway. General, our data assistance the notion that curcumin induces the apoptosis of human monocytic leukemia THP 1 cells by means of the activa tion of JNK ERK Jun pathways.