massiliensis strain JC225T with those of C. flavigena strain definitely 134T  and C. fimi strain ATCC 484T (EMBL accession number “type”:”entrez-nucleotide”,”attrs”:”text”:”CP002666″,”term_id”:”332337569″,”term_text”:”CP002666″CP002666). The draft genome sequence of C. massiliensis has a smaller size than those of C. flavigena and C. fimi (3.40 vs 4.12 and 4.26 Mb, respectively), a lower G+C content (71.22 vs 74.3 and 74.7, respectively), and a smaller number of predicted genes (3,131 vs 3,788 and 3,863, respectively). In addition, C. massiliensis shared a mean 88.75% (range 70.01-100%) and 89.61% (range 70.07-100%) sequence similarity with C. flavigena and C. fimi, respectively, at the genome level. Conclusion On the basis of phenotypic, phylogenetic and genomic analyses, we formally propose the creation of Cellulomonas massiliensis sp.
nov. that contains the strain JC225T. This bacterium has been found in Senegal. Description of Cellulomonas massiliensis sp. nov. Cellulomonas massiliensis (ma.si.li.e��n.sis. L. gen. masc. n. massiliensis, of Massilia, the Latin name of Marseille where was isolated C. massiliensis). Colonies are transparent and smooth with a diameter of 1 mm on blood-enriched Columbia agar and Brain Heart Infusion (BHI) agar. Cells are rod-shaped with a diameter and length ranging from 0.37 to 0.60 ��m (mean of 0.48 ��m), and from 0.55 to 1.4 ��m (mean of 0.95 ��m), respectively. Optimal growth is achieved aerobically. Weak growth is observed with 5% CO2 and under microaerophilic conditions. No growth is observed under anaerobic conditions.
Growth occurs between 30-37��C, with optimal growth at 37��C. Cells stain Gram-positive, are non-endospore forming, and are motile. Catalase, oxidase, aesculin hydrolysis and ��-galactosidase activities are present. Indole production, nitrate reduction, glucose fermentation, arginine dihydrolase, urease, gelatin hydrolysis, and glucose, arabinose, mannose, mannitol N-acetyl-glucosamine, maltose, gluconate, caprate, adipate, malate, citrate, and phenyl-acetate assimilation activities are absent. Cells are susceptible to amoxicillin, imipenem, ciprofloxacin and gentamicin, but resistant to trimethoprim/sulfamethoxazole and metronidazole.
The 16S rRNA and genome sequences are deposited in Genbank and EMBL under accession numbers “type”:”entrez-nucleotide”,”attrs”:”text”:”JN657218″,”term_id”:”573033269″,”term_text”:”JN657218″JN657218 and “type”:”entrez-nucleotide”,”attrs”:”text”:”CAHD00000000″,”term_id”:”390167473″,”term_text”:”CAHD00000000″CAHD00000000, respectively. The G+C content of the genome is 71.22%. The type strain JC225T (= CSUR P160 = DSM 25695) was isolated from the fecal flora of a healthy patient in Senegal. Acknowledgements The authors thank Mr. Julien Batimastat Paganini at Xegen Company for automating the genomic annotation process.
Kurthia massiliensis strain JC30T (CSUR 141T = DSM 24639T) is the type strain of K. massiliensis sp. nov.