Blades or white roses in s w Chsh Usern used. Transgenic plants expressing the 35S: embroidered PtrDFR2 transcription cassette or vector: transcription cassette PtrDFR1 produces much darker than the pink flowers were on the wild-type control or transgenic plants that either the observed 35S Flt Signaling pBI121. Zus Tzlich anthocyanin pigments extracted from the corolla of flowers of various plants, and roughly measured by spectrophotometry. With respect to either a transgenic and wild-type or pBI121 vector control plants was significantly embroidered h Higher accumulation of anthocyanins in transgenic plants overexpressing PtrDFR1 detected. No transgenic plants showed a significant increase PtrDFR2 such.
RT-PCR analysis demonstrated overexpression or PtrDFR1 PtrDFR2 correlated in each line Tivozanib of transgenic tobacco. Taken together, these results suggest that the protein may PtrDFR1 with endogenous enzymes of the anthocyanin biosynthetic pathway in tobacco and the result of the increase in anthocyanin accumulation interact in vivo, w PtrDFR2 while the protein does not seem to have such a function. Our results are in line with an earlier report entered in the M. truncatula MtDFR1 overexpression in transgenic tobacco plants Born in a visible increase in the accumulation of anthocyanins flowers, w During MtDFR2 not, indicating that unexpected properties and differences lie in two orthologous proteins DFR. Functional characterization of genes for the biosynthesis of transgenic poplars PtrDFR TB has been shown that CT concentrations are as high as 50% in poplar trees and up to 18% dry weight in the Bl Ttern of aspen.
CT accumulation of herbivores and pathogens in LZEN go Induced, suggesting that TC play an r Important in the defense and protection against biotic stresses. To date, the biosynthetic pathways for TC and is large number of polyphenolic compounds is well established. DFR encodes a key enzyme in the sp More advanced stages of the synthesis of CT in plants, and entered the overexpression of a gene in the forage legume Lotus corniculatus DFR Born changing levels of CT. To determine the function of the in the biosynthetic pathway PtrDFRs CT, their ORF flanked by the 35S promoter, were introduced into S. tomentosa Carr. More than 20 independent-dependent kanamycin-resistant transgenic lines harboring either p35S: PtrDFR1 or p35S: PtrDFR2 expression cassettes were produced.
All transgenic plants was best by PCR analysis using gene-specific primers CONFIRMS. No significant difference between morphological transgenic plants and wild-type poplars observed. Three lines that each construct were Selected for further analysis Hlt. Spectrophotometric quantification of total TC showed that all transgenic lines, which carried out the 35S: 35S or PtrDFR1: PtrDFR2 transcription cassettes one Erh increase CT content in leaf tissue showed in comparison to wild-type plants and contr transgenic vector control pBI121. In 35S: PtrDFR1 lines 4 and 12, an increase of three times the height H of the CT was found in leaf tissue to control compared to the wild type. Similar 35S: PtrDFR2 lines, the concentration of TC Total lines 5 and 9 more than twice as healing.