Figure S3

Figure S3 BMPR-IB inhibited the subcutaneous growth of glioblastoma cells. A) The subcutaneous models of nude glioblastoma cells, which over-expressed of BMPR-IB and knocked down BMPR-IB. B) The tumor masses derived from the subcutaneous

xenograft. C) H&E staining of tumors derived from subcutaneous xenografts of glioblastoma cells. N: Normal connective tissue; T: Glioblastoma tissue. Figure S4 Quantitative check details analysis of CD34 positive microvessels in the glioblastoma specimens. Glioblastoma ABT 737 specimens that were derived from U251-C/U251-IB and SF763-si-Con/SF763-si-IB cells were stained by CD34 using immunohistochemistry method. Error bars represent SD (performed selleck products in triplicate). *p < 0.01. Table S1 Primer sequences for p21, p27, p53, CDK2, CDK4, Skp2, BMPR-IB (human) and GAPDH. (DOC 4 MB) References 1. Maher EA, Furnari FB, Bachoo RM, et al.: Malignant glioma: genetics and biology of a grave matter. Genes Dev 2001, 15:1311–1333.PubMedCrossRef 2. Gonzalez J, de Groot J: Combination therapy for malignant glioma based on PTEN status. Expert Rev Anticancer Ther 2008, 8:1767–1779.PubMedCrossRef 3. Ye F, Gao Q, Cai MJ: Therapeutic targeting of EGFR in malignant gliomas. Expert

Opin Ther Targets 2010, 14:303–316.PubMedCrossRef 4. Folkins C, Man S, Xu P, et al.: Anticancer therapies combining antiangiogenic and tumor cell cytotoxic effects reduce the tumor-like cell fraction in glioma xenograft tumors.

Cancer Res 2007, 67:3560–3564.PubMedCrossRef 5. Liu S, Tian Z, Yin F, Fan W, Fan M: Expression and Functional Roles of Smad1/5/8 and BMPR-IB in glioma development. Cancer Investig 2009, 27:734–740.CrossRef 6. Hogan BL: Bone morphogenetic proteins: multifunctional regulators of vertebrate development. Genes Dev 1996, 10:1580–1594.PubMedCrossRef 7. Tanabe Y, Jessell TM: Diversity and pattern in the developing Arachidonate 15-lipoxygenase spinal cord. Science 1996, 274:1115–1123.PubMedCrossRef 8. Massagué J: TGF-β signaling: receptors, transducers, and Mad proteins. Cell 1996, 85:947–950.PubMedCrossRef 9. Mehler MF, Kessler JA: Cytokines and neuronal differentiation. Crit Rev Neurobiol 1995, 9:419–446.PubMed 10. Hoodless P, Haerry T, Abdollah S, et al.: MADR1, a MAD-related protein that functions in BMP2 signaling pathways. Cell 1996, 85:489–500.PubMedCrossRef 11. Imamura T, Takase M, Nishihara A, et al.: Smad6 inhibits signalling by the TGF-beta superfamily. Nature 1997, 9:622–626. 12. Hayashi H, Abdollah S, Qiu Y, et al.: The MAD-related protein Smad7 associates with the TGF-β receptor and functions as an antagonist of TGF-β signaling. Cell 1997, 89:1165–1173.PubMedCrossRef 13. Nakashima K, Yanagisawa M, Arakawa H, et al.: Synergistic signaling in fetal brain by STAT3-Smad1 complex bridged by p300. Science 1999, 284:479–482.PubMedCrossRef 14.

Leave a Reply

Your email address will not be published. Required fields are marked *


You may use these HTML tags and attributes: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <strike> <strong>