Bioinformatics 2004,20(17):3246–3248.PubMedCrossRef 44. Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R, Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI, et al.: QIIME allows analysis of high-throughput community sequencing data. Nat Meth 2010,7(5):335–336.CrossRef 45. Lee SG, Kim CM, Hwang KS: Development of a software Roscovitine price tool for in silico simulation of Escherichia coli using a visual programming

environment. J Biotechnol 2005,119(1):87–92.PubMedCrossRef 46. Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, GS-9973 nmr Summers MD: Virus Taxonomy: Sizth Report of the International Committee on Taxonomy of Viruses. New York: Springer-Verlag;

1995. Vol. Supplement 10 47. Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, Kulam-Syed-Mohideen AS, McGarrell DM, Marsh T, Garrity GM, Tiedje JM: The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res 2009, 37:D141-D145.learn more PubMedCentralPubMedCrossRef 48. Whiteley AS, Jenkins S, Waite I, Kresoje N, Payne H, Mullan B, Allcock R, O’Donnell A: Microbial 16S rRNA Ion Tag and community metagenome sequencing using the Ion Torrent (PGM) Platform. J Microbiol Methods 2012,91(1):80–88.PubMedCrossRef 49. Edgar RC: Search and clustering orders

of magnitude faster than BLAST. Bioinformatics 2010,26(19):2460–2461.PubMedCrossRef 50. Caporaso JG, Bittinger K, Bushman FD, DeSantis TZ, Andersen GL, Knight R: PyNAST: a flexible tool for aligning sequences to a template alignment. Bioinformatics 2010,26(2):266–267.PubMedCentralPubMedCrossRef 51. DeSantis TZ, Hugenholtz P, Larsen N, Rojas M, Brodie EL, Keller K, Huber T, Dalevi D, Hu P, Andersen GL: Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl Environ Microbiol 2006,72(7):5069–5072.PubMedCentralPubMedCrossRef 52. Li W, Godzik A: Cd-hit: a fast program for clustering cAMP and comparing large sets of protein or nucleotide sequences. Bioinformatics 2006,22(13):1658–1659.PubMedCrossRef 53. Price MN, Dehal PS, Arkin AP: FastTree: computing large minimum evolution trees with profiles instead of a distance matrix. Mol Biol Evol 2009,26(7):1641–1650.PubMedCentralPubMedCrossRef 54. Wang Q, Garrity GM, Tiedje JM, Cole JR: Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 2007,73(16):5261–5267.PubMedCentralPubMedCrossRef 55. Lozupone C, Hamady M, Knight R: UniFrac–an online tool for comparing microbial community diversity in a phylogenetic context. BMC Bioinforma 2006, 7:371.CrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions Conceived and designed experiments: DTP.